2019-09-05 09:35:082025-05-13 23:39:33
description
ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer
ATP-dependent DNA helicase, coupling of DNA replication, transcription, recombination and segregation, facilitates unwinding of ICEBs1 DNA for horizontal transfer
function
plasmid rolling-circle replication, conjugative transfer of ICEBs1
coupling of DNA replication, transcription, recombination and segregation
Gene
Phenotypes of a mutant
essential [Pubmed|12682299]
essential [Pubmed|12682299]
inactivation can be suppressed by inactivation of either [[gene|recA]], [[gene|recJ]], [[gene|recO]], or [[gene|mfd]] [pubmed|32793628]
The protein
Catalyzed reaction/ biological activity
PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop [Pubmed|20723756]
dismantles [[protein|RecA]] filaments [Pubmed|20723756]
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
coupling of DNA replication, transcription, recombination and segregation [pubmed|32793628]
PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop [Pubmed|20723756]
dismantles [[protein|RecA]] filaments [Pubmed|20723756]
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
The protein
[SW|Domains]
two RecA-like domains [Pubmed|24147116]
intrinxically disordered C-terminal domain (for interaction with [SW|RNA polymerase]) [Pubmed|24147116]
two RecA-like domains [Pubmed|24147116]
intrinxically disordered C-terminal domain (for interaction with [SW|RNA polymerase]) [Pubmed|24147116]
[SW|UvrD-like helicase ATP-binding domain](aa 10-289) (according to UniProt)
[SW|UvrD-like helicase C-terminal] (aa 290-570) (according to UniProt)
References
Original Publications